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2 | Rubisco quantitation kit

Каталожный номер
AS09 409
Производитель
Хозяин
Primary antibody: Rabbit. Secondary antibody: Goat.
Клональность
Иммуноген
KLH-conjugated synthetic peptide was used to elicit anti-RbcL antibody. No baculovirus was used in production of this product.
Количество
1 x 50 µl of AS03 037, RbcL | Rubisco large subunit, form I and form II (amount enough for 50-100 Western blots)1 x 100 µl of AS01 017S, Rubisco protein standard (0.15 pmoles / µl, amount enough for generation of standard curve in 85assays (standard curve
Условия хранения
RbcL antibody and protein standard: Please store at -20
Дополнительная информация
Quantitative western blot: detailed method description, video tutorialDiscussion over some critical aspects of quantitative western blot: Heidebrecht et al. (2009). Improved semiquantitative Western blot technique with increased quantification range. J.
Разбавление
1 : 5000-1 : 10000 (WB)
Подтвержденное взаимодействие
Arabidopsis thaliana, Chlamydomonas reinhardtii, Cyanophora paradoxa, Cynara cardunculus var altilis, Emiliana huxleyi, Euglena gracilis, Gonyaulax polyedra, Heterosigma akashiwo, Micromonas pusila, Porphyra sp. Spinacia oleracea, Synechococcus PCC 7942,
Ориентировочные молекулярный вес
Rubisco protein standard: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii)
Предполагаемое взаимодействие
di and monocots, conifers, mosses, liverworts, welwitschia, green algae, red alge, brown algae, cryptomonad, cyanobacteria including prochlorophytes, gamma-proeobacteria, beta-proteobacteria, alpha proteobacteria
Происхождение
The Rubisco quantitation kit is suitable for quantitation of Rubisco in plant and algal samples using quantitative immunoblotting. It contains anti-RbcL antibody, Rubisco protein standard and the Protein Extraction Buffer for quantitative protein extracti
Область применения
131 266,99 руб.

Описание

Recommended secondary antibody: AS09 602, Goat anti-rabbit IgG (H&L), HRP conjugated

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